Summaries of published peptide research for qualified researchers
Summary of the enzymatic recycling assay for reduced and oxidised glutathione as described by Tietze in 1969 and in a 2006 Nature Protocols article, with a 2009 overview of chromatographic and electrophoretic separation methods.
Read studySummary of three 2001 papers that identified the peptides encoded by the KiSS-1 gene as ligands of the G protein-coupled receptor GPR54 and measured binding affinity, phosphoinositide hydrolysis, calcium mobilisation and MAP kinase phosphorylation in transfected CHO-K1 cells.
Read studySummary of 1998 and 1999 studies on the 37-residue cathelicidin peptide LL-37, covering the anion- and concentration-dependent coil-to-helix transition, pH dependence, oligomerisation and the orientation of the helix on lipid membranes.
Read studySummary of two published methods for measuring nicotinamide adenine dinucleotide in cell and tissue extracts, a reverse-phase HPLC method and a targeted LC-MS method, with the 1961 paper on the acid and alkali stability of pyridine nucleotides.
Read studySummary of a 2001 study that measured NGF and BDNF mRNA in glial cultures from newborn rat basal forebrain after exposure to the heptapeptide Semax, with a 2006 radioligand binding study on rat basal forebrain membranes.
Read studySummary of a 2016 rat study that profiled 84 neurotransmission-related genes in frontal cortex one and three hours after Selank or GABA at 300 ug/kg, with a 2017 follow-up in IMR-32 cells.
Read studySummary of a 2018 study showing that the mitochondrial-encoded 16-residue peptide MOTS-c moved to the nucleus of cultured cells under nutrient restriction in an AMPK-dependent manner and associated with ARE-regulating transcription factors, with the 2015 paper that first described the peptide.
Read studySummary of a 2011 study that followed fluorescein-labelled Ala-Glu-Asp-Gly into the cytoplasm and nucleus of HeLa cells and measured Stern-Volmer quenching constants for its binding to labelled deoxyribooligonucleotides and DNA.
Read studySummary of the cell-culture arm of a 2008 study in which the tripeptide Lys-Pro-Val was examined for PepT1-mediated uptake and for effects on NF-kB reporter activity and cytokine secretion in Caco2-BBE, HT29-Cl.19A and Jurkat cells.
Read studySummary of structural, thermodynamic and kinetic studies of the Cu(II) complex of Gly-His-Lys, covering X-ray and solution structures, the conditional dissociation constant by ITC, and interpeptidic copper exchange rates.
Read studySummary of a 1997 study that measured Boyden chamber chemotaxis, scratch-assay migration and metalloproteinase secretion in human umbilical vein endothelial cells exposed to thymosin beta-4, with the 1991 actin-binding characterisation.
Read studySummary of a 2011 cell-culture study that measured explant outgrowth, transwell migration, F-actin staining and FAK-paxillin phosphorylation in rat tendon fibroblasts exposed to BPC-157.
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